野生欧李生长期各组织器官中不同形态钙的分配率变化

以野生欧李为试材,逐级提取不同形态的钙,研究欧李生长发育阶段(萌动期-开花期-果实发育期)主要结果基生枝各组织器官(木质部、韧皮部、花和果实、新梢)中不同形态钙(水溶钙、果胶钙、磷酸钙、草酸钙、剩余钙)的分配率变化及其与总钙分配的相关关系。结果表明:不同组织器官中钙吸收和分配与钙形态转化和分配密切相关,随欧李生长期发育进程,木质部和韧皮部中不同形态钙分配呈明显下降趋势,新梢中不同形态钙分配呈明显增长趋势,花器官中水溶钙、果胶钙和磷酸钙形态在盛花期均有一分配高峰,果实中果胶钙和水溶钙形态分配分别在果实发育初期和后期有一分配高峰;花和果实总钙分配率变化与输导组织器官木质部和韧皮部中各种形态钙的分配呈显著或极显著正相关关系,新梢总钙分配率变化与输导组织器官木质部和韧皮部中各种形态钙的分配呈显著或极显著负相关关系。欧李生长期结果基生枝各组织器官中存在钙形态转化与再分配,水溶钙和果胶钙形态是欧李果实钙素吸收积累的主要钙形态。     

季风常绿阔叶林不同恢复阶段附生维管植物多样性及分布

通过对云南普洱地区不同恢复阶段(恢复15年、30年和原始林群落)季风常绿阔叶林群落内附生维管植物的调查,分析了不同恢复阶段附生维管植物的物种丰富度、多度、分布和相似性关系,并探讨附生维管植物与宿主的关系。结果表明:(1)在0.81 hm²的调查样地中,共发现附生维管植物3 116株(分属9科20属22种);(2)原始林中附生维管植物的物种丰富度(17种)要显著高于恢复30年(7种)和15年(5种)群落;(3)原始林与不同恢复阶段群落中附生维管植物的物种组成有显著不同,恢复15年群落中的附生植物主要由蕨类植物组成,兰科植物是原始林附生植物的主要物种组成,有些物种仅出现在原始林中,可以作为季风常绿阔叶林恢复程度的指示物种;(4)原始林附生维管植物的多度要显著高于恢复阶段;(5)3个群落类型中的附生维管植物在水平方向都为聚集分布,恢复15年群落主要分布在0~5 m,原始林中分布可达到20 m;(6)原始林中附生维管植物物种丰富度和多度与宿主的胸径之间存在显著正相关,而2个恢复阶段森林中不存在相关性。     

不同土地利用方式下渭-库绿洲土壤盐渍化特征分析

基于渭干河—库车河三角洲绿洲土壤盐渍化现象发展的趋势,采用经典统计分析方法,对研究区不同土地利用方式下的土壤电导率、总盐量和pH值进行描述性统计分析,通过单因素方差方法对不同土地利用方式下土壤盐分指标进行差异显著性检验,并利用最小显著极差法(LSD)进行多重比较。研究结果表明:在不同土地利用方式和土层深度下,土壤电导率和总盐量的平均水平差异显著(p0.05),其含量由高到低依次为:荒漠灌丛盐碱草地耕地林地,而土壤pH值则无显著差异,土壤盐分呈现出明显的表层富集现象。本研究的结论对于提高此区域农业生产潜力和盐碱地综合治理具有重要的理论和实际意义。     

Studies on Nutrient Distribution in Some Zambian Soils with Special Reference to Sulphur Using GIS (Geographic Information Systems) I. Total Sulphur Distribution in Major Zambian Soils

Total sulphur content of some benchmark soils was evaluated by correlating with the contents of soil organic C and amorphous oxides (Al and Fe), and the particle size distribution. An equation model to calculate the content of total soil sulphur was first obtained by regression analysis. Introducing these chemical and physical data of all the map units except for the wetlands into the equation model, the sulphur level on a national scale was estimated for each soil mapping unit in the exploratory soil map of Zambia. Second, the total sulphur content was rated into 3 classes based on the frequency distribution of the total sulphur content in the benchmark soils. Finally, the rated total sulphur content was incorporated into the national soil map database of GIS to produce a map with the total sulphur content of Zambian soils. The extent of each class was calculated and the distribution of the areas was examined in relation to rainfall and soil formation.     

A Novel Particle Contact Assay with the Yeast Saccharomyces cerevisiae (8 pp)

Goal, Scope and Background   Numerous xenobiotics released into surface waters are transferred to suspended particulate matter and finally attached to sediments. Aquatic organisms may be exposed to them by direct particle feeding, by physical contact with contaminated surfaces as an exposure route, and by the uptake of dissolved contaminants after equilibration via the free water phase. In order to assess potential sediment toxicity, each of these exposure routes has to be addressed. This paper presents a newly developed particle contact assay that uses the fermentation performance of a specific Saccharomyces cerevisiae strain for the assessment of toxic effects in sediments. The test procedure is based on the characteristic feature of growing yeast cells to attach to sediment particles, which are also relevant for the accumulation of contaminants. The physical contact with lipophilic contaminants mirrors an exposition pathway for the direct uptake into the cells. In order to quantitatively characterize the toxic effects of particle attached pollutants on the fermentation performance, unpolluted native reference sediment was spiked with representatives for widely distributed anthropogenic contaminants. Methods   Saccharomyces cerevisiae was established as sensitive eukaryotic microorganism for the ecotoxicological assessment of particle attached anthropogenic contaminants in freshwater sediments. For this purpose, yeast cells were cultivated in sediment samples and the resulting fermentation performance was continuously measured. Sediments artifically spiked with HCB, PCB, g-HCH, DDT, and benzo(a)pyrene and solutions of each contaminant were comparatively investigated by means of their adverse effects on yeast fermentation performance. Additionally, four native river sediments characterized by increasing levels of pollution were assessed by the yeast particle contact assay, and simultaneously by standard aquatic tests with algae, daphniae, and luminescent bacteria using pore water and elutriates. Results of the bioassays were related to specific sediment contamination with respect to metals and organic priority pollutants. Results and Discussion   In sediments spiked with PCB and benzo(a)pyrene fermentation, performance was affected extensively below concentrations inhibiting fermentation in contaminant solutions. This suggests a high efficiency of the exposure route by physical contact. The fermentation performance was only slightly affected by single lipophilic pollutants, whereas mixtures of individually spiked sediments caused critically reduced fermentation performance suggesting additive synergistic effects. Native river sediments modestly to critically polluted by hazardous organic compounds lead to a slightly to dangerously reduced fermentation performance in the yeast contact assay. These inhibitory effects were much less pronounced in the standard bioassays conducted with algae, daphniae and luminescent bacteria, applying pore waters and elutriates as sample matrices. Using pore water, inhibition was measured only in the most polluted sediment, elutriates lead to a slight inhibition of the algal growth in the undiluted sample only. These results indicate an improved sensitivity of the yeast particle contact assay compared to the standard assays, due to uptake and physical cell contact as additional routes of exposure. Conclusion   The yeast particle contact assay is a valuable tool for the assessment of ecotoxicological potential in freshwater sediments. Since the assay addresses physical contact as an exposure route, it indicates bioavailability of lipophilic compounds in sediments. Outlook   The sensitive indication of bioavailable contaminants associated to sediment particles by the newly developed yeast particle contact assay recommends it as a complementary microbial bioassay in a test battery for assessing major pathways of contaminants in whole sediments.     

荒漠-绿洲边缘区生态过渡带的土壤颗粒分形特征

通过实地采样和实验室分析,测定了黑河中游荒漠绿洲边缘区生态过渡带的土壤粒级分布、有机碳(SOC)、全氮(TN)、全磷(TP)、速效氮(AN)和速效磷(AP)的含量,并采用1993年杨培岭等提出的用粒径的重量分布代替数量分布的土壤分形模型,计算了土壤颗粒分形维数;最后利用统计分析法,研究了颗粒分形维数与土壤粒级分布及各养分指标的关系.结果表明:从荒漠边缘向绿洲延伸的过渡带,土壤中砂粒含量占绝对优势,粉粒居中,黏粒最少,采样区土壤质地较粗;土壤颗粒分形维数在2.105～2.609之间变动,处于极低水平,与土壤各养分指标具有相似的变化趋势,除靠近绿洲区存在一个"生态裂谷"外,整体呈上升趋势;利用相关分析法得出,土壤颗粒分形维数与砂粒含量呈显著负相关,与黏粉粒及各养分指标均呈显著的正相关;利用回归分析法进行定量分析,砂粒含量每增加1g/kg,颗粒分形维数D降低0.0019个单位;粉粒和黏粒含量每增加1g/kg,颗粒分形维数D分别增加0.002和0,023个单位,对土壤颗粒分形维数变化幅度的影响依次为黏粒>粉粒>砂粒,分形维数对黏粒含量的变化最为敏感.因此,可以将土壤颗粒分形维数作为衡量荒漠-绿洲边缘区土壤养分变化状况的指标之一,用于反映荒漠绿洲区土壤的退化状况.     

不同种植模式下紫色土养分流失影响因子研究

通过建立5个不同种植模式的径流小区，对每个小区径流泥沙中养分的含量进行研究．探讨机械组成、微团聚体含量、粘粒团聚度等因子对养分流失影响的程度，结果发现：15～30cm土层中〈0．01mm的粘粒含量与径流泥沙中全钾含量和速效钾含量有显著的相关性。0～15cm层中的〈0．01mm和〈0．001mm微团聚体含量都与径流泥沙中有机质、速效钾和全氮的含量有显著的相关性。同层中〈0．01mm粘粒团聚度与径流泥沙中全氮含量和速效钾含量有显著的相关性，而同层〈0．001mm粘粒团聚度与径流泥沙中全磷含量、速效钾含量和速效磷含量有显著的正相关。     

去除养殖水体悬浮颗粒的多向流重力沉淀装置设计及性能

为了高效去除循环水养殖系统中的固体悬浮颗粒物,研发了多向流重力沉淀装置,并对其水力特性及相关结构参数进行试验研究。该文以固体悬浮颗粒物去除率为试验指标,设计了5～40 min的水力停留时间（hydraulic retention time,HRT）及斜管填料规格参数的双因素试验。结果显示,随着HRT增加,多向流重力沉淀装置的固体悬浮颗粒物去除率呈显著上升的阶段性变化特征,但以HRT为20 min时沉淀装置的综合处理效果最优,最高平均去除率可达（58.57 %±10.12 %）,其中粒径小于20 μm的微颗粒去除率为19.5%,粒径大于60 μm的微颗粒去除率高达90.3 %;较小的斜管直径可显著提高多向流重力沉淀装置的固体悬浮颗粒物去除率,而斜管高度对去除率无显著影响。多向流重力沉淀装置用于去除循环水养殖系统中的固体悬浮颗粒物,是一种行之有效的方法。     

基于粒子图像测速技术的液力变矩器涡轮内流场测试与分析

液力变矩器是自动变速器的主要部件,其在乘用车、载重汽车、公共汽车和机车上的应用广泛。液力变矩器内部流动特性影响其外部性能,为了深入研究其内部流动特性,基于粒子图像测速(particle image velocimetry,PIV)技术对液力变矩器涡轮内流场进行试验研究。采用帧频为1 000帧/s的CCD高速相机,在不同工况下(输入与输出的转速比分别取0、0.3、0.5、0.7)采集不同粒子直径、不同粒子浓度下的流动图像,经过图像预处理,对连续两帧图像进行互相关计算,获得涡轮径向切面的速度场和涡量场。通过对流场分布结构和流动区域上复杂流动现象的对比分析,发现投入流场中粒子浓度越高(1 500mL蒸馏水中投入2.4 g粒子)、粒子直径越小(10μm)时,识别并提取流动区域上的流动参数越丰富,流速场和涡量场信息越可靠。液力变矩器内部高梯度流场分布结构和非均匀流速场分布导致出现多尺度涡旋和反向流等复杂流动现象,造成液力变矩器内部流动能量损耗,随转速比提高,涡轮流场结构趋于规律,能耗逐渐降低。该文试验测量与分析结果对于液力变矩器结构优化和性能提升提供了参考。     

超微粉碎对桑叶粉理化性质的影响

为拓展超微粉碎技术在食品添加剂和药品开发中应用,以桑叶为原料,分别对桑叶粗粉进行干法超微粉碎2 、4、6、8 h,得到4种不同粒径大小的桑叶超微粉,并对桑叶粗粉与4种超微粉的理化性质进行比较。研究结果表明,超微粉碎能明显减小桑叶粗粉的颗粒粒径（P＜0.05）,随着超微粉碎时间的增加,超微粉的粒径减小,粉体的粒径分布范围变窄,比表面积和体积密度显著增加（P＜0.05）,休止角、滑角和膨胀性显著降低（P＜0.05）,水溶性/持水力、持油力、蛋白质的水溶性和多糖的水溶解性均有不同程度的增加,但多酚含量变化不显著（P＞0.05）。扫描电镜结果显示,粗粉经超微粉碎后,其颗粒呈零散的分布形态。说明超微粉碎可改善桑叶粉的部分理化性质,可将这些性质应用于食品添加剂和常规食品开发与加工中。所得研究结果将为超微粉碎技术在桑叶食品中工业化应用提供数据支持和理论依据。